目的 研究蛋白酶体激活因子γ（REGγ）在人肺癌组织及细胞系中的表达以及其对肺癌细胞增殖、细胞周期以及迁移能力等多种恶性生物学行为的影响。方法 免疫组织化学染色检测REGγ在肺癌组织以及癌旁正常组织中的表达情况；Western blot 检测肺癌细胞系及肺正常上皮细胞系中REGγ的表达；构建REGγ过表达及沉默肺癌H1975稳定细胞株，用MTT法检测不同REGγ表达情况的肺癌细胞H1975稳定株的增殖变化；PI染色流式检测REGγ对肺癌细胞周期的作用；通过细胞划痕实验观察REGγ对肺癌细胞迁移能力的影响。结果 在肺癌组织中REGγ的表达程度明显高于癌旁正常组织。在多个肺癌细胞系中，REGγ表达量均高于正常细胞系。REGγ过表达可以促进肺癌细胞增殖（P<0.05），增加肿瘤细胞S+G2/M期的比例（P <0.05），并增强其迁移能力（P <0.05），沉默REGγ后上述影响均发生逆转。结论 REGγ对肺癌多种恶性生物学行为有明显促进作用。
英文摘要:
【Abstract】 Objective To determine the expression of proteasome aotivator γ (REGγ) in human lung cancer tissues and cell lines and its association with malignant biological behaviors. Methods Immunohistochemistry (IHC) was used to detect the expression of REGγ in lung cancer and normal lung tissues. The expressions of REGγ in lung cancer cells and normal epithelial cells were determined by Western blot. The H1975 lung cancer stable cell lines with different levels of \REGγ expression were constructed and their proliferations were evaluated by MTT assay. PI staining was used to assess the influence of REGγ on cell growth cycle. The effect of REGγ on the migration of lung cancer cells were observed with the cell scratch experiment. Results Lung cancer tissues had significantly higher levels of REGγ expression than normal tissues. Similarly, lung cancer cell lines showed higher levels of REGγ expression than the normal epithelial cell line. The overexpression of \REGγ enhanced cancer cell proliferations (\P<0.05), promoted more cells into the S+G \2/M phase (\P<0.05) and promoted the migration of cancer cells (\P<0.05). All of these effects were reversed after suppression of \REGγ. Conclusion REGγ facilitates malignant biological behaviors of lung cancers.
