目的 回顾性分析四川大学华西医院2000年与2010年收治的原发性支气管肺癌患者的临床流行病学特征及病理类型分布，以初步了解肺癌的流行趋势。方法 收集2000年与2010年四川大学华西医院收治的初诊为原发性支气管肺癌并登记为四川地区常住人口的病例，对两组患者的性别、年龄、城乡来源、吸烟史、职业暴露、病理类型等临床资料进行对比分析。结果 收集肺癌病例共2 167例，其中2000年616例，2010年1 551例。肺癌患者男女比由2000年的2.78∶1下降至2010年的2.13∶1（P =0.013）。2000年与2010年肺癌患者发病年龄差异无统计学意义（P =0.302）。地域分布上，十年间大城市及中小城市肺癌患者构成比下降（大城市: 42.1% vs. 32.0%, P <0.001; 中小城市: 39.9% vs. 31.7%, P<0.001)，乡镇及农村患者构成比上升（乡镇: 5.5% vs. 8.1%, P=0.041；农村: 12.5% vs. 28.2%, P <0.001）。病理类型分布方面，2000年肺癌患者病理类型以鳞癌为主，2010年以腺癌为主，十年间鳞癌构成比由44.8%下降至28.7%（P<0.001），腺癌构成比由43.0%升至53.1%（P<0.001），小细胞癌构成比由3.7%升至11.9%（P<0.001）；两个时间段鳞癌在男性患者中的构成比均高于女性（2000年: 52.1% vs. 24.5%; 2010年: 37.7% vs. 9.5%），而腺癌在女性患者中的构成比均高于男性（2000年：60.7% vs. 36.6%; 2010年: 75.8% vs. 42.9%）；两个时间段鳞癌在老年患者（≥60岁）中的构成比均高于青年患者（<45岁）（2000年: 50.5% vs. 33.8%; 2010年: 30.2% vs. 15.6%），而腺癌在青年患者中的构成比均高于老年患者（2000年: 54.9% vs. 36.9%; 2010年: 57.1% vs. 51.8%）；2000年与2010年肺癌吸烟患者病理类型均以鳞癌为主（55.6%, 40.9%），非吸烟患者均以腺癌为主（58.4%, 75.7%）；伴职业暴露的肺癌患者病理类型均以腺癌为主（46.2%, 60.2%）。结论 近十年来，肺癌女性患者构成比明显上升，腺癌及小细胞癌构成比明显上升，鳞癌与男性、老年患者（≥60岁）及吸烟密切相关，腺癌则与女性、青年患者（<45岁）及职业暴露密切相关。
英文摘要:
【Abstract】 Objective To construct the sh-rpS6 lentivirus vector targeting ribosomal protein S6 (rpS6) and explore its effect on proliferation in lung adenocarcinoma A549 cell lines. Methods Sequences targeting the rpS6 gene were selected. The double strand shRNA oligo was ligated to pGCsil-GFP lentivirus vector and transformed into E.coli. The resulting recombinant vector was verified by sequencing. After transfection and lentivirus packing, the viral particles were collected and infected A549 cells. After selection of GFP positive cells by FACS, mRNA and protein expression levels of rpS6 were determined by real time PCR and Western blot. In the following experiment, the proliferation changes of A549 cell lines after the interference by sh-rpS6 was investigated by using CCK-8 kit. Results The sequencing result confirmed that pGCsil-sh-rpS6-GFP vector was successfully developed. Stably transfected A549 cell lines by sh-rpS6 were selected through FACS, with a selection ratio of 86.80%. The silencing effects of sh-rpS6 were determined by real time PCR and Western blot, suggesting that mRNA and protein expression of rpS6 in the targeted cells reduced by (79.72±6.83)% and （83.77±12.13)%, significantly lower than those of control groups. In vitro showed the cell proliferation with sh-rpS6 was significantly slower than that of controls (P<0.05). Conclusion The constructed sh-rpS6 lentivirus vector could inhibit the expression of rpS6 in A549 cell lines effectively and significantly slow the cell proliferation In vitro
