非诺贝特对体外卵巢癌细胞SKOV3生长及迁移的作用

Effects of Fenofibrate on the Growth and Migration of Ovarian Cancer Cells in vitro

中文关键词:  卵巢癌 非诺贝特 过氧化物酶增殖物激活受体 增殖 凋亡 细胞迁移

英文关键词:Ovarian cancer Fenofibrate Peroxisome proliferator-activated receptors Proliferation Apoptosis Migration

中文摘要:

      目的 初步了解过氧化物酶增殖物激活受体α（PPARs)激动剂非诺贝特对卵巢癌细胞生长情况的影响。方法 选用人卵巢癌SKOV3细胞株作为研究对象，选择不同浓度的非诺贝特进行培养，设置对照组（不含非诺贝特），采用MTT法测定不同浓度非诺贝特对体外卵巢癌SKOV3细胞系细胞增殖抑制作用；荧光显微镜观察Hoechst/碘化丙啶(PI)及Annexin-Ⅴ/PI染色后非诺贝特诱导SKOV3细胞凋亡作用；细胞划痕实验观察非诺贝特作用后SKOV3细胞的迁移改变。结果 ①MTT实验中发现，与对照组相比，10、25、50、75、100 μmol/L非诺贝特处理体外卵巢癌SKOV3细胞24、48、72 h后，各浓度对SKOV3细胞均产生抑制作用（P<0.05）。并随浓度增加，抑制效果增强，24、48、72 h 100 μmol/L抑制率最高（P<0.05)，分别为55.72%±0.28%、57.63%±0.47%、72.41%±0.62%（P<0.05）。②Hoechst/PI和Annexin-Ⅴ/PI荧光染色置荧光显微镜下检测示，10、25、50、75、100 μmol/L非诺贝特处理细胞24 h后，诱导细胞凋亡，随着非诺贝特药物浓度的增加，SKOV3细胞凋亡越明显。③划痕损伤实验结果显示，与对照组相比，在10、25、50、75、100 μmol/L非诺贝特作用于SKOV3细胞24 h后，细胞迁移距离缩短（P<0.05）。结论 非诺贝特对体外人卵巢癌SKOV3细胞的增殖及细胞迁移均有抑制作用，并一定程度上诱导细胞凋亡。

英文摘要:

      Objective To investigate the effects of fenofibrate, a lipid-lowering drug, on the growth and migration of human ovarian cancer cells SKOV3 in vitro. Methods A human ovarian cancer cell line (SKOV3) as the research object, was incubated with serum-free media for 24 h. These cells were then treated by appropriate concentrations of fenofibrate for different time, including control and experimental groups. Cell proliferation was evaluated by MTT assay. Apoptosis was detected by Hoechst/PI and Annexin-Ⅴ/PI fluorescent assay. The migration of cells was measured by the scratch-wound healing assay. Results The MTT assay results demonstrated that the fenofibrate (10, 25, 50, 75, 100 μmol/L) could inhibit the proliferation of SKOV3 cells after 24, 48 and 72 h treatment (P<0.05). The inhibition rate for 24, 48, 72 h-treatment was 55.72%±0.28%, 57.63%±0.47%, 72.41%±0.62% respectively (P<0.05). The effects increased with the concentrations. Hoechst/PI and Annexin-Ⅴ/PI fluorescent assay showed that after stimulus for 24 h, fenofibrate induced apoptosis of SKOV3 cells in a concentration-dependent manner was observed. A significant inhibited cells migration distance (P<0.05) evaluated with scratch-wound healing assay was observed after treatment with fenofibrate (10, 25, 50, 75, 100 μmol/L) for 24 h. Conclusion Lipid-lowering drug fenofibrate can inhibit the growth and migration of human ovarian cancer cell SKOV3 in vitro, to some extent induce apoptosis. But the detailed mechanism need to be further studied.