柱前紫外衍生-高效液相色谱法同时测定功能食品中八种糖醇和糖

Simultaneous Determination of Sugar Alcohols and Sugars in Functional Foods by Precolumn Ultraviolet Derivatization High Performance Liquid Chromatography

中文关键词:  糖醇 苯甲酰氯 柱前紫外衍生 高效液相色谱法 功能食品

英文关键词:Sugar alcohols Benzoyl chloride Precolumn ultraviolet derivatization High performance liquid chromatography Functional foods

中文摘要:

      目的 建立功能食品中赤藓糖醇、木糖醇、半乳糖醇、山梨醇、甘露醇、麦芽糖醇，以及葡萄糖和三氯蔗糖的柱前紫外衍生-高效液相色谱同时测定方法。 方法 食品中的糖醇等经水提取后，与苯甲酰氯反应生成紫外衍生物，衍生物经C₁₈柱分离，柱温为30 ℃，甲醇-水梯度洗脱，流速1.00 mL/min，检测波长为232 nm。 结果 所测8种物质的标准曲线线性良好（r>0.999），检出限低于2.2 μg/mL，加标回收率为89.6%~117.0%，日内相对标准偏差小于5%。 结论 该方法衍生反应简便快速、所需试剂经济易得，本法不需特殊检测器，易于推广，适用于功能食品中多种糖醇的同时测定。

英文摘要:

      Objective To establish a method using precolumn ultraviolet derivatization coupled with high performance liquid chromatography (HPLC) for simultaneous determination of erythritol, xylitol, galactitol, sorbitol, mannitol, maltitol, glucose and sucrose in functional foods. Methods Target sugar alcohols and sugars in food samples were extracted in water by ultrasonic method and then reacted with benzoyl chloride to form violet-absorbing products, which were separated on a C₁₈ column with gradient elution using methanol and water as mobile phase. The experiment was performed using a flow rate of 1.00 mL/min, column temperature at 30 ℃ and detected wavelength at 232 nm. Results The linear correlation coefficients of all the derivatives were more than 0.999.The detection limits of the method were as low as 2.2 μg/mL. The average recoveries were 89.6%-117.0%, with intraday relative standard derivations lower than 5%. Conclusion This method is simple, inexpensive and easy to operate and it is suitable for the determination of sugar alcohols and glucose and sucrose in functional foods.