骨髓间充质干细胞移植治疗急性心肌梗死的多模态体内示踪分子成像

Bone Mesenchymal Stem Cell Transplantation for Acute Myocardial Infarction: in vivo Tracing with Multi-modality Molecular Imaging

中文关键词:  PEI2k-SPIO 萤火虫荧光素酶 骨髓间充质干细胞 生物发光 磁共振成像

英文关键词:PEI2k-SPIO Luciferase Bone mesenchymal stem cell Bioluminescence Magnetic resonance imaging

中文摘要:

      目的 利用多聚乙烯-超顺磁性氧化铁(PEI2k-SPIO)标记带有萤火虫荧光素酶（Luciferase）的SD大鼠骨髓间充质干细胞（BMSCs/Luciferase），探索多模态成像活体示踪BMSCs移植治疗急性心肌梗死的可行性。方法 PEI2k-SPIO成功标记BMSCs/Luciferase细胞后行普鲁士兰染色及MTT验证标记的有效性及安全性。超声引导下原位移植至SD大鼠急性心肌梗死模型的梗死心肌边缘，分别在移植后1 d和1周行磁共振成像（MRI）及移植后1 d、2 d、3 d和1周行光学成像。结果 MTT结果显示PEI2k-SPIO标记的BMSCs/Luciferase细胞与未标记BMSCs/Luciferase细胞间存活细胞率差异无统计学意义(P<0.05)。MRI在细胞移植后1 d能观察到注射区域低信号，移植后1周未观察到低信号影；移植后1、2、3 d可同时探测到生物发光，移植后7 d未检测到生物发光。结论 多模态分子成像可同时示踪移植干细胞的位置和判断细胞存活状态。

英文摘要:

      Objective To investigate the feasibility of tracking bone mesenchymal stem cell (BMSCs) dual-labeled with polyethylenimine 2k-superparamagnetic iron oxide (PEI2k-SPIO) and Luciferase transplantation for acute myocardial infarction in vivo by using magnetic resonance imaging (MRI) and fluorescence imaging. Methods BMSCs/Luciferase was incubated with culture medium containing PEI2k-SPIO for 24 h. Prussian-blue staining and MTT were used to assess the efficacy and safety of labeling with PEI2k-SPIO. Guided with echocardiography, the dual-labeled BMSCs were injected into the margin of infarction myocardium. MRI and fluorescence imaging were performed to monitor the cells in vivo at different times (1,2,3,7 d). Results As demonstrated by MTT, there was no significant difference in survival rate between the labeled and unlabeled cells (P>0.05). Within a week after transplantation, all PEI2k-SPIO-labeled BMSCs showed a significant decreased signal on MRI. Dual-labeled BMSCs were detected bioluminescence with fluorescence imaging, but disappeared after one week. Conclusion Multi-modality imaging can not only trace the location of labeled BMSCs but also demonstrate the survival of labeled BMSCs in vivo.