GNB3 C825T和ACE I/D多态性相互作用与子痫前期关系的研究

Interaction Between GNB3 C825T and ACE I/D Polymorphisms in Pre-eclampsia

中文关键词:  子痫前期 基因多态性 GNB3 ACE

英文关键词:Preeclampsia Polymorphism GNB3 ACE

中文摘要:

      目的 探讨G蛋白β3亚单位（GNB3）和血管紧张素Ⅰ转换酶（ACE）基因相互作用是否与子痫前期的发生有关联。方法 采用ACE基因PCR扩增片段长度多态性及GNB3基因限制性片段长度多态性方法，对成都地区汉族188例子痫前期及273例正常孕妇进行对照检测。结果 子痫前期及正常孕妇GNB3C825T和ACE I/D位点基因型和等位基因频率在两组之间差异无统计学意义（P>0.05），未见GNB3 C825T等位基因及ACEI/D相互作用与子痫前期发生的危险增加有关（OR 0.439~1.203，P均>0.05）。对联合基因型与血压水平关系的分析发现，正常对照组TT/II基因型携带者其舒张压水平增高〔（77.61±1.26） mmHg(1 mmHg=0.133 kPa)〕，且高于TT/ID〔（70.94±1.64） mmHg〕、CT/ID〔（73.15±0.89） mmHg〕和CT/DD〔（72.57±2.14） mmHg〕基因型携带者（P均<0.05）；未见患者组联合基因型与收缩压和舒张压水平有关（P>0.05）。结论 本研究未发现GNB3基因C825T及ACE基因I/D位点的相互作用与中国人（成都地区）子痫前期发病有关联，但发现两基因的相互作用与正常孕妇舒张压水平有关。

英文摘要:

      Objective To verify the hypothesis if interaction between the G protein β3 subunit (GNB3) C825T polymorphism and angiotensin-I converting enzyme (ACE) insertion/deletion (I/D) could lead to the increased risk of pre-eclampsia. Methods Analyses of ACEand GNB3genotypes were performed in 188 pre-eclamptic patients and 273 normal pregnant controls by polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism in Chinese population, respectively. Results The distributions of alleles and genotypes for the GNB3C825T and ACE I/D polymorphisms were not found to be significantly associathed with pre-eclamptic status. No significant interaction of the influence of GNB3T allele and ACEgenotypes on the risk of pre-eclampsia was observed (OR 0.439-1.203, all P >0.05). However, we found that in homozygous 825T genotype carriers with the ACE II genotype in controls diastolic blood pressure (DBP) levels showed highest 〔(77.61±1.26） mmHg (1 mmHg=0.133 kPa)〕 among other three genotype combinations 〔TT/ID, （70.94±1.64） mmHg; CT/ID, （73.15±0.89） mmHg; CT/DD, （72.57±2.14） mmHg〕 (all P <0.05). No significant effect on systolic blood pressure (SBP) or DBP levels in the patients were observed. Conclusion Our data suggest no significant interaction of the GNB3825T allele carriers with theACEI/D polymorphism in pre-eclampsia in Chinese population in Chengdu area. However there is the interaction of the two genes on DBP levels in pregnancy women without pre-eclampsia in the population.