肿瘤相关成纤维细胞在垂体腺瘤侵袭性行为中的作用研究

The Role of Cancer-associated Fibroblasts in Invasive Behavior of Pituitary Adenoma

中文关键词:  肿瘤相关成纤维细胞 垂体腺瘤 侵袭性 MMP-9

英文关键词:Cancer-associated fibroblasts Pituitary adenomas Invasion MMP-9

中文摘要:

      目的 初步研究肿瘤相关成纤维细胞在垂体腺瘤侵袭性行为中的作用。方法 依据术前磁共振（MRI）检查和术中观察，收集典型侵袭性（IPA组，n=7）和非侵袭性（nIPA组,n=8）垂体腺瘤新鲜组织，进行原代培养肿瘤相关成纤维细胞，并用肌动蛋白α（α-SMA）染色鉴定；利用Transwell迁移和侵袭性试验比较两组细胞的迁移和侵袭能力，并用免疫细胞化学、Western blot比较两组肿瘤相关成纤维细胞α-SMA、基质金属蛋白酶（MMP）-9的表达差异。结果 新鲜垂体腺瘤组织中培养出α-SMA阳性的肿瘤相关成纤维细胞，两组细胞的迁移能力无明显差异，侵袭能力IPA组强于nIPA组（ P=0.010）；IPA组细胞MMP-9表达高于nIPA组（ P=0.025），α-SMA的表达在两组之间差异无统计学意义。结论 肿瘤相关成纤维细胞可能通过分泌更多的MMP-9增强自身侵袭能力，为肿瘤细胞侵袭周围组织提供条件。

英文摘要:

      Objective To determine the role of cancer associated fibroblasts (CAFs) in the invasive behavior of pituitary adenoma. Methods Pituitary adenoma tissues were divided into invasive group (IPA) and non-invasive group (nIPA) according to pre-operative MRI and observations during surgery. Those tissues were cultured and CAFs were identified through α smooth muscle actin (α-SMA). The migratory and invasive ability of CAFs was tested with transwell migration and invasion assay. The expressions of α-SMA and matrix metalloproteinase (MMP)-9 from CAFs were determined by immunocytochemistry and Western blot. Results All cultured CAFs expressed α-SMA. No significant difference in migratory ability of CAFs was found between the IPA and nIPA tissues; however, CAFs from the IPA tissues had stronger invasive ability than those from the nIPA tissues ( P=0.010). Higher levels of MMP-9 expression were found in group IPA as compared with nIPA ( P=0.025). No significant difference in the expression of α-SMA was found between the two groups. Conclusion CAFs may promote invasive behavior by secreting more MMP-9, which may play a part in the invasive behavior of pituitary adenomas.