正常结直肠组织、腺瘤、结直肠腺癌中miRNAs的表达差异

目的探讨6种microRNA(miRNA)在正常结直肠组织、管状腺瘤、管状绒毛状腺瘤及腺癌中的表达差异。方法共搜集44例管状腺瘤、16例管状绒毛状腺瘤、20例腺癌患者的病变组织及其旁正常组织标本，应用Real-time PCR方法分别检测miR-1、miR-9、miR-31、miR-135a、miR-137、miR-145共6种miRNA的表达量，分析表达差异。结果 ①管状腺瘤中miR-1、miR-9、miR-137、miR-145的表达均低于瘤旁正常组织，miR-31、miR-135a的表达均高于瘤旁正常组织，其中miR-9、miR-135a差异有统计学意义（ P<0.05），miR-1、miR-137、miR-145、miR-31差异无统计学意义（ P>0.05）。②管状绒毛状腺瘤中miR-1、miR-137、miR-145的表达均低于瘤旁正常组织，miR-9、miR-135a、miR-31的表达均高于瘤旁正常组织,差异均有统计学意义（ P<0.05）。③结直肠腺癌中miR-1、miR-9、miR-137、miR-145的表达均低于癌旁正常组织，miR-31、miR-135a的表达高于癌旁正常组织，差异均有统计学意义（ P<0.05）。结论正常结直肠组织、结直肠腺瘤、结直肠癌中miRNA的差异表达，提示上述miRNA在从正常结直肠组织到结直肠腺瘤、结直肠腺瘤到结直肠癌的发生发展过程中可能发挥作用，为miRNA作为疾病预测、预后指标提供了实验基础。

英文摘要:

Objective To determine the oxidative stress level in peripheral blood of vanadium-exposed workers, as an indication of population health effect of vanadium on human neurobehavioral system. Methods 86 vanadium-exposed workers and 65 non-exposed workers were recruited by cluster sampling. A questionnaire was administered to collect demographic and occupational exposure information. Serum activity of superoxide dismutase（SOD）, inducible nitric oxide synthase (iNOS ) and malonaldehyde （MDA） contents were detected by kit assay. The differences in oxidative stress level between vanadium-exposed and non-exposed workers were compared. Results Vanadium-exposed workers had higher levels of MDA contents than the controls. The total superoxide dismutase（T-SOD）activity in vanadium-exposed workers was significantly lower than that in the controls, which was associated with lowered levels of manganese superoxide dismutase (Mn-SOD) activity. No changes in serum levels of cupro-zinc superoxide dismutase (CuZn-SOD) was found in vanadium-exposed workers. No difference in iNOS activity was found between vanadium-exposed workers and controls. Conclusion Vanadium exposure increases free radical production in serum and reduces antioxidant capacity. But the relationship between vanadium exposure and iNOS damage remains uncertain.

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