TAO患者眼眶CD90＋和CD90－成纤维细胞亚群脂成纤维细胞转化的研究

The Study of Lipofibroblasts Differentiation of CD90＋ and CD90－ Orbital Fibroblasts Subsets in Patients with TAO

中文关键词:  甲状腺相关性眼病 眼眶成纤维细胞亚群 脂成纤维细胞

英文关键词:Thyroid-associated ophthalmopathy Orbital fibroblasts subsets Lipofibroblast

中文摘要:

      目的 比较甲状腺相关性眼病患者（TAO）眼外肌来源和眶脂肪/结缔组织来源CD90^＋和CD90^－的成纤维细胞诱导转化为脂成纤维细胞的转化率，明确对脂肪形成起主要作用的成纤维细胞亚群。方法 免疫磁分离法分离TAO患者眼眶CD90^＋和CD90^－的成纤维细胞，诱导CD90^＋和CD90^－成纤维细胞亚群转化为脂成纤维细胞，分别于诱导后第5、10、15、20、25 d油红O染色，计数染色阳性细胞，计算脂成纤维细胞的转化率。结果 TAO患者眼外肌来源的成纤维细胞转化为脂成纤维细胞的转化率低于眼眶脂肪/结缔组织来源的成纤维细胞（P<0.05）。CD90^＋成纤维细胞转化为脂成纤维细胞的转化率低于CD90^－成纤维细胞（P<0.05）。眼外肌来源CD90^＋成纤维细胞基本不能转化为脂成纤维细胞。眼眶脂肪/结缔组织来源的CD90^－成纤维细胞转化率最高（P<0.05）。结论 眼眶成纤维细胞具有分化为脂肪细胞功能的脂成纤维细胞主要是CD90^－的眶脂肪/结缔组织成纤维细胞。

英文摘要:

      Objective Divided orbital fibroblasts from patients with thyroid-associated ophthalmopathy (TAO) into CD90^＋ and CD90^－ subsets respect to surface CD90 expression, then determined whether CD90^＋ and/or CD90^－ fibroblasts were capable of differentiating into lipofibroblasts. Methods Fibroblasts subset separation into CD90^＋ and CD90^－ subsets was accomplished by three to four rounds of magnetic bead selection, then treated with ３-isobutyl-1-lmethylxanthine (IBMX), insulin and dexamethasone which was an known inducer of the lipofibroblastic phenotype, then the cells were observed every day to find Lipid droplets. Oil red O staining were conducted at 5 d, 10 d, 15 d, 20 d and 25 d after inducing. The percent of lipofibroblasts were calculated. Results The ratio in fibroblast derived from extraocular muscles of differentiating into lipofibroblast is less than from connective/adipose tissue (P<0.05). The ratio of CD90^＋ fibroblast is less than CD90^－ fibroblast (P<0.05). CD90^＋ cells derived from extraocular miscles could not be induced to differentiate into lipofibroblast. The ratio in CD90^－ fibroblast from connective/adipose tissue is highest (P<0.05). Conclusion Orbital fibroblast which has the function of differentiating into lipofibroblast is mainly CD90^－ connective/adipose tissue.