目的 研究高迁移率族蛋白N2（high mobility group chromosomal protein N2,HMGN2）对宫颈癌裸鼠移植瘤生长的抑制作用。方法 建立宫颈癌裸鼠移植瘤模型，随机分为阴性对照组、HMGN2蛋白组、顺铂组和联合用药组，分别予生理盐水、HMGN2蛋白、顺铂、HMGN2蛋白加顺铂隔日腹腔注射。观察肿瘤的生长情况，经过4次给药后处死裸鼠，取出瘤块，测量其体积计算抑瘤率，并行苏木精-伊红（HE）染色观察形态学变化。结果 成功建立宫颈癌裸鼠移植瘤模型。第4次给药后，HMGN2蛋白组、顺铂组、联合用药组肿瘤体积平均值分别为（0.14±0.07） cm3、（0.11±0.06） cm3 、（0.11±0.07） cm3，与阴性对照组〔（0.38±0.12） cm3〕相比差异有统计学意义（P <0.05）；3组肿瘤体积平均值的差异无统计学意义（P >0.05）。 HMGN2蛋白组、顺铂组、联合用药组肿瘤抑制率分别为0.62±0.18、0.71±0.17、0.70±0.18，差异无统计学意义（P >0.05)。肿瘤经肉眼观察可见阴性对照组瘤块较大，HE染色可见HMGN2蛋白组、顺铂组和联合用药组细胞坏死较阴性对照组明显。结论 HMGN2 蛋白可以明显抑制宫颈癌裸鼠移植瘤的生长。
英文摘要:
Objective To study the effect of high mobility group chromosomal protein N2 on inhibiting cervical cancer in nude mice. Methods Model of cervical cancer were established in nude mice. They were randomly divided into 4 groups including negative control group, HMGN2 group, cisplatin group and HMGN2 with cisplatin group. After 4 injections, the tumor size were calculated and tumor tissues were stained by haematoxylin eosin (HE) staining. Results Transplated tumor models were established successfully. The tumor sizes of negative control group 〔(0.38±0.12） cm3〕 were significantly lower than those of HMGN2 group 〔(0.14±0.07）cm3, cisplatin group 〔(0.11±0.06） cm3〕 and HMGN2 combined with cisplatin group 〔(0.11±0.07） cm3〕. No differences were detected in HMGN2 group, cisplatin group and HMGN2 with cisplatin group in tumor sizes. The tumor inhibition rates of HMGN2 group, cisplatin group and HMGN2 with cisplatin group were 0.62±0.18, 0.71±0.17 and 0.70±0.18, respectively. The necrosis area were smaller in negative control group than in other three groups by HE staining. Conclusion HMGN2 has a significant inhibitory effect on transplanted cervical cancer in nude mice.
