MACC1、Snail和KISS-1蛋白在浸润性乳腺癌中的表达及其临床病理意义

Expressions of MACC1, Snail, and KISS-1 Proteins in Infiltrating Breast Carcinoma and Its Clinicopathological Features

中文关键词:  乳腺肿瘤 结肠癌转移相关基因1 Snail KISS-1 上皮间质转化

英文关键词:Breast neoplasm Metastasis-associated in colon cancer 1 Snail KISS-1 Epithelial-mesenchymal transition

中文摘要:

      目的 探讨结肠癌转移相关基因1（MACC1）和抑癌基因KISS-1在浸润性乳腺癌（IBC）中的蛋白表达及其与上皮间质转化相关因子Snail表达的关系和临床病理意义。方法 通过免疫组织化学法检测250例IBC（IBC组）和80例正常乳腺组织（对照组）中MACC1、KISS-1和Snail的表达情况，分析临床病理资料特征，评估对患者预后的影响。结果 在对照组中，MACC1、KISS-1和Snail的阳性表达率分别为7.5%、87.5%和5.0%；在IBC组中，MACC1、KISS-1和Snail阳性表达率分别为63.6%、38.0%和58.8%，两组之间差异均有统计学意义（P <0.05）。MACC1、KISS-1和Snail蛋白的表达在IBC患者的淋巴结转移与否、肿瘤组织的不同分化程度、不同临床分期、不同分型之间差异均有统计学意义（P <0.05）。MACC1和Snail蛋白阳性表达组患者总的生存时间低于其阴性表达组患者（P <0.001）；KISS-1表达阳性的患者其生存时间高于其阴性组患者（P <0.001）。Cox回归分析结果表明，MACC1、Snail蛋白的阳性表达和KISS-1的阴性表达是影响IBC患者术后生存时间的独立危险因素（P <0.05)。结论 MACC1、Snail和KISS-1的反常表达参与了IBC的浸润及转移过程，早期联合检测MACC1、Snail和KISS-1的表达对IBC的进展及预后判定均有重要意义。

英文摘要:

      Objective To investigate the protein expressions of metastasis-associated in colon cancer 1 (MACC1), KISS-1 (a cancer ruppressor gene) and Snail (the marker of epithelial-mesenchymal transition) in infiltrating breast carcinoma (IBC) and explore the role of them in invasion, metastasis and prognosis in IBC. Methods Expressions of MACC1, Snail and KISS-1 were examined by immunohistochemistry in 250 specimens of IBC and 80 specimens of normal breast tissues. Their clinicopathological features were analyzed, and their influence on patients’ survival was identified. Results The positive rate of MACC1, Snail and KISS-1 in normal breast tissues and IBC tissues was 7.5%, 5.0%, 87.5% and 63.6%, 58.8%, 38.0%, respectively. And there was a significant difference between the IBC group and control group (P<0.05). The positive expressions of MACC1, Snail and KISS-1 were significantly different in different TNM stages, lymph node metastasis, types and grades of tumor (P<0.05). The survival time of positive KISS-1 group was significantly longer than that of negative group (P<0.001); the survival time was significantly shorter in positive MACC1 group or Snail group than that of negative groups (both P<0.001). Cox regression analysis indicated that the positive expressions of MACC1, Snail and negative expression of KISS-1 were independent risk factors of IBC (P<0.05). Conclusion Abnormal expression of MACC1, Snail and KISS-1 should be involved in the invasion and metastasis of IBC. The combined detection in the expressions of MACC1, Snail and KISS-1 at the early stage may play an important role in predicting the progression and prognosis of IBC.