芍药苷对MRL/lpr狼疮小鼠肺损伤的保护作用

目的探讨芍药苷对MRL/lpr狼疮小鼠肺损伤的影响。方法将系统性红斑狼疮MRL/lpr小鼠随机分为MRL/lpr组、MRL/lpr+地塞米松 (1.5 mg/kg) 组，MRL/lpr+芍药苷(20 mg/kg)组，MRL/lpr+芍药苷 (40 mg/kg) 组，每组10只，给药7 d；另取10只野生型C57BL/6小鼠作为正常对照组。第8天采用酶联免疫吸附法（ELISA）检测小鼠血清中超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和血清炎症因子白细胞介素6（IL-6）、白细胞介素1β（IL-1β）及肿瘤坏死因子α（TNF-α）水平；采用HE染色检测肺部病理改变，采用蛋白质免疫印迹（Western blot）技术检测各组小鼠肺组织中磷酸化磷脂酰肌醇-3激酶（P-PI3K）、磷酸化丝氨酸苏氨酸蛋白激酶（P-Akt）、磷酸化核因子κB p65(P-NF-κBp65)、磷酸化NF-κB抑制蛋白α（P-IκBα）蛋白的表达。结果芍药苷能显著降低血清MDA含量；提高血清SOD、CAT、GSH-PX水平，减少血清炎症因子水平，改善肺组织病理改变，并能显著抑制P-PI3K、P-Akt、P-NF-κBp65、P-IκBα蛋白表达。结论芍药苷对MRL/lpr狼疮小鼠肺损伤起到保护作用。

英文摘要:

ObjectiveTo investigate the protective effects of paeoniflorin on the lung injury in systemic lupus erythematosus with mouse model. Methods Ten wild type mice and 40 MRL/lpr mice were used in this stuy. MRL/lpr mice were randomly assigned to MRL/lpr group, MRL/lpr + dexamethasone (1.5 mg/kg) group, MRL/lpr + paeoniflorin (20 mg/kg) group, and MRL/lpr + paeoniflorin (40 mg/kg). The levels of malondialdehyde（MDA），superoxide dismutase (SOD）, catalase (CAT）, glutathione peroxidase (GSH-Px） in serum were detected. The serum levesl of inflammatory cytokines were measured. Lung pathological changes were determined by HE staining. The protein level of phospho-phosphatidylinositol-3 kinase (P-PI3K), phospho-serine-threonine kinase B(P-Akt）, phospho-nuclear factor kappa B (P-NF-κB), phospho-inhibitor of nuclear factor kappa Bα (P-IκBα) were detected by Western blot. Results Paeoniflorin decreased serum level of MDA and increased the levels of SOD, CAT, GSH-PX, and decreased the levels of inflammatory cytokines. Paeoniflorin improved lung pathological changes and inhibited the protein levels of P-PI3K, P-Akt, P-NF-κBp65, and P-IκBα in the lung tissue of MRL/lpr mice. Conclusion Paeoniflorin may be beneficial for the prevention of lung injury in systemic lupus erythematosus.

查看全文查看/发表评论下载PDF阅读器

关闭